Contact

Professional Status

Employed
Open to opportunities

About Me

Student graduated with a PhD in veterinary science and a Masters in Applied Microbiology. Currently a Posdoctoral scholar at University of California, Santa Cruz

Experience

Postdoctoral Scholar

Univeristy of California, Santa Cruz
Since November 2016
  • Helicobacter pylori: Critical role of biofilm in gastric colonization and inflammation
  • Representative member of UCSC Stem Postdocs Association (since 2017)

Supervising summer student (University of California, Santa Cruz)

University of California, Santa Cruz
Since 2017
  • Screening H. pylori biofilm mutant from a mutagenesis bank
  • Biofilm assay
  • PCR and sequencing

Teaching course microbial pathogenesis

Université de Montréal
September 2015 to December 2015
  • Teaching course microbial pathogenesis 2 (Master’s degree, veterinary science)
    Part_1. Microbial envelope
    Part_2. Surface polysaccharides

Webmaster

www.bacterialbiofilms.com
2014 to 2016

Supervising student internship

Groupe de recherche sur les maladies infectieuses du porc (GREMIP)
January 2014 to May 2014
  • Study the effect of sub-inhibitory doses of antibiotics on APP biofilms
  • Confocal microscopy and TEM
  • expression gene by qPCR
  • Immunodetections of PGA
  • A manuscript was published

Microbiology Research Internship

Groupe de recherche sur les maladies infectieuses du porc (GREMIP)
Since April 2011
  • Site-directed mutagenesis (allelic exchange)
  • Immunodetection of extracellular polymeric substance (EPS)
  • EPS extraction (eDNA...)
  • Biofilms culture in static and dynamic conditions
  • Drip flow reactor
  • Enzymatic treatments on biofilms
  • Immunodot tests
  • Macrophages in vivo Infection
  • PBMC isolation
  • Lipid A extractions and structure analysis
  • Microscopies

Microbiology Research Internship

Centre National de Greffe de Moelle Osseuse, Faculté de médecine de Tunis
October 2009 to June 2010
  • Antibiotic susceptibility tests and serotyping assay of P. aeruginosa clincal strains
  • Static biofilm formation and enzymatic treatments
  • Curcumin and berberine chloride test on biofilm
  • Biofilm genes identification
  • Swarming et twitching motility

Microbiology Research Internship

Institut de Biologie Moléculaire et Médecine, Université Libre de Bruxelles
January 2009 to June 2009
  • E. coli chromosomal toxin antitoxin systems identification by PCR
  • Sequencing
  • Phylogenetic analyses

Library assistant

Université de Montréal
Since September 2012
  • Assist students and teachers in location of print and non-print resouces
  • Check books and materials in and out to students and teachers using the computerized circulation system
  • Monitor student use of the library
  • Assist in maintaining the appearance of the library

Practical class demonstrator

Université de Montréal Faculty of Veterinary Medicine
Since January 2012
  • Responsible for practical work (microbiology), students first cycle of Veterinary Medicine

Proctor

Université de Montréal
Since September 2012
  • Monitoring students during the writing of tests/exams

Skills

  • Microscopic identification, biochemical testing of bacteria
  • Antibiotic susceptibility tests and serotyping assay
  • Biofilms culture in static and dynamic conditions
  • Enzymatic and chemicals treatments on biofilms
  • Swarming et twitching motility tests
  • Drip flow reactor systems
  • MBEC assay
  • Cell culture
  • Macrophages culture
  • Cytokines detections
  • PBMC isolation and proliferation
  • Nitric oxide quantification
  • Biofilm staining
  • Biomass and thickness
  • EPS detection
  • Immunodetection of extracellular polymeric substance (EPS)
  • Site-directed mutagenesis
  • DNA and RNA extraction
  • EPS extraction (eDNA...)
  • PCR and RT-PCR
  • Lipid A micro-extractions
  • LPS extractions

Education

PhD in Veterinary Science, Microbiology and Infectious Disease

Faculté de médecine vétérinaire, Université de Montréal
May 2011 to September 2016

Study of micro-organisms in their natural environment. Development of techniques for culturing bacteria form biofilms. Effect of antibiotics on biofilm. Immune response towards biofilms. Phenotypic changes in biofilms cells.
GPA 3.85/4.3

1_Actinbacillus pleuropneumoniae biofilms induce lower response of porcine alveolar macrophages. S. Hathroubi, C. Provost, C. A. Gagnon and M. Jacques. Innate immunity
(http://www.ncbi.nlm.nih.gov/pubmed/27226465)

2_Biofilms: Bacterial shelters against antibiotics. S. Hathroubi, M.A Mekni, P. Domenico, D. Nguyen and M. Jacques. Microbial Drug Resistance
(http://www.ncbi.nlm.nih.gov/pubmed/27214143)

3_Surface polysaccharide mutants reveal that absence of O antigen reduces biofilm formation of Actinobacillus pleuropneumoniae. S. Hathroubi, M.A. Hancock, J.T. Bossé, P.R. Langford, Y.D.N Tremblay, J. Labrie and M. Jacques. 2015. Infection and Immunity

(http://www.ncbi.nlm.nih.gov/pubmed/26483403).

4_Sub-inhibitory concentrations of penicillin G induce biofilm formation by field isolates of Actinobacillus pleuropneumoniae. S. Hathroubi, S-È. Fontaine-Gosselin, Y.D.N. Tremblay, J. Labrie and M. Jacques. 2015. Vet Microbiology (http://www.ncbi.nlm.nih.gov/pubmed/26130517).

5_Les biofilms bactériens : leur importance en santé animale et en santé publique. Y.D.N. Tremblay, S. Hathroubi and M. Jacques. 2014. Canadian Journal of Veterinary Research (http://www.ncbi.nlm.nih.gov/pubmed/24688172).

Certificate '' Bacteria biofilms in chronic infections''

Faculty of Health Sciences, University of Copenhagen
September 2012 to November 2012

Distinguish biofilms from planktonic bacteria both in nature and in medical related situations. Examine health care problems caused by infections involving bacterial biofilms. Design and develop biofilm models. Strategies for future research.

Training/Certification program for animal experimentation

Université de Montréal
Since September 2011

The Ethics of animal experimentation gives me the right to experiment on animals ifor laboratory research

Master Applied Microbiology, Environment and Health

Université Pierre Et Marie Curie, Paris 6, France
September 2008 to June 2010

A good understanding of microbiology ; bacteriology, virology, parasitology, environmental quality and health.
Project communication: GENOTYPIC AND PHENOTYPIC CHARACTERIZATION OF BIOFILM PRODUCTION IN PSEUDOMONAS AEROGINOSA STRAINS IN THE BONE MARROW TRANSPLANT CENTER OF TUNIS, TUNISIA

Bachelor of Life Sciences

Université Pierre et Marie Curie, Paris 6 France
September 2005 to June 2008

Scientific knowledge of biology and the general organisation of the animal and vegetable kingdoms as well as the functioning of organisms, from the molecular level to integrated biological systems.

Interests

  • Cloud mining
  • Trading Ethereum
  • Video games, Photography
  • Photo treatment Photoshop, ACDSee
  • 16 countries visited
  • Road trips cross-country (USA and Canada)